FIG 6.

Fe-S cluster assembly is diminished but essential in BS. (A) Samples containing the same numbers of cells were prepared from the PS and BS cells and analyzed by Western blotting. Nfs and Isu are expressed at 45% and 22%, respectively, in the BS compared to the PS. Enolase was used as a loading control. (B) Growth of the BS Isu RNAi cells in the absence (solid line) or presence (dashed line) of tetracycline was examined. After the induction, a significant growth defect was observed. The efficiency of RNAi was verified by Western blotting using anti-Isu antibody and enolase as a loading control (inset).