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Unrooted phylogenetic reconstruction of relationships between 440 Brucella isolates representing distinct species and biovars by extended MLSA. The tree was constructed with the concatenated sequence data of the 21 loci (>10.2 kb) using the neighbor-joining algorithm with the Jukes-Cantor model. Brucella species are distinguished by different colors. Strains M1, M2, T, and K investigated in this study are designated by arrows (24; Whatmore, unpublished data).
