TABLE 4.
Kinetic parameters of SDOsa
| SDO | Vmax (μmol O2 min−1 mg−1) | kcat (s−1) | Km (μM) | kcat/Km (mM−1 s−1) |
|---|---|---|---|---|
| PaSdoA | 10.9 ± 2.1 | 5.8 | 342 ± 192 | 17.0 |
| PpSdoA | 11.9 ± 0.3 | 6.5 | 150 ± 72 | 43.3 |
| BxSdoA | 7.2 ± 1.2 | 3.8 | 204 ± 102 | 18.6 |
| AtBlh | 4. 6 ± 1.0 | 3.6 | 108 ± 84 | 33.3 |
| SmBlh | 5.7 ± 1.0 | 4.5 | 168 ± 90 | 26.8 |
| MxETHE1a | 90.3 ± 13.3 | 38.5 | 132 ± 72 | 291.7 |
| MxETHE1b | 38.6 ± 2.5 | 16.5 | 138 ± 32 | 119.6 |
| MxETHE1c | 63.1 ± 6.0 | 26.6 | 248 ± 60 | 107.3 |
| CnETHE1 | 6.7 ± 0.4 | 3.0 | 108 ± 24 | 27.8 |
| CnSdoA | 23.2 ± 3.1 | 12.8 | 336 ± 132 | 38.1 |
| CnSdoA D217G | 0.25 ± 0.01 | 0.14 | 78 ± 2 | 1.8 |
| CnSdoA D217N | 1.5 ± 0.2 | 0.8 | 258 ± 114 | 3.1 |
a
Kinetic analysis was done using 100 mM KPi buffer (pH 7.4) at 25°C with varying concentrations of GSSH. The kinetic parameters with standard errors were obtained by using the GraFit 5 software program.