TABLE 1.
Pattern no. or speciesa | No. of isolates with pattern | Catalase activity | Hippurate hydrolysis | Sensitivity tob: |
Biochemical phenotypec | No. of isolates amplified for 16S rRNAd | Isolate(s) identified by 16S rRNAe (%) | |
---|---|---|---|---|---|---|---|---|
Nalidixic acid | Cephalothin | |||||||
1 | 1 | − | − | S | R | Campylobacter spp. | 1 | Arcobacter (100 [n = 1]) |
2 | 3 | + | − | R | R | Campylobacter spp. | 3 | C. lari (100 [n = 3]) |
3 | 4 | + | − | S | R | Campylobacter spp. | 3 | C. jejuni (67), C. lari (33 [n = 3]) |
4 | 5 | + | + | R | R | C. jejuni | 4 | C. jejuni (100 [n = 4]) |
5 | 71 | + | + | S | R | C. jejuni | 59 | C. jejuni (100 [n = 59]) |
C. jejuni (control) | 1 | + | + | S | R | C. jejuni | 1 | C. jejuni (100) |
C. coli (control) | 1 | + | − | S | R | C. coli | 1 | C. coli (100) |
Five different patterns emerged from biochemical testing of isolates. Isolates were compared to known C. jejuni and C. coli controls.
S, susceptible; R, resistant.
Phenotypes were declared based on biochemical tests.
Some isolates could not be amplified by PCR.
The 16S rRNA gene was analyzed phylogenetically, and isolates were identified to the genus and species levels based upon their relationship (close proximity with support values) with known reference sequences.