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. 2014 Mar;80(5):1750–1762. doi: 10.1128/AEM.03654-13

FIG 1.

FIG 1

NrdH reduces disulfide bonds by the TrxR/NADPH pathway but not the MSH/Mtr/NADPH pathway. (A to C) Reduction of RNR (A), insulin (B), and DTNB (C) by NrdH (10 μM) coupled to the TrxR/NADPH regeneration system. Trx (10 μM) was used as a positive control. Negative controls are the omission of TrxR in the presence of NrdH (−Control 1), omission of TrxR in the presence of Trx (−Control 2), and omission of both Trx and NrdH in the presence of TrxR (−Control 3). (D to F) Reduction of RNR (D), insulin (E), and DTNB (F) by NrdH (10 μM) coupled to the MSH/Mtr/NADPH regeneration system. Mrx1 (10 μM) was used as a positive control. Negative controls are the omission of Mtr in the presence of NrdH (−Control 1), omission of Mtr in the presence of Mrx1 (−Control 2), omission of both Mrx1 and NrdH in the presence of Mtr (−Control 3), and omission of Mtr in the presence of Mrx1 and NrdH (−Control 4). The reduction of RNR (A and D) and insulin (B and E) were recorded by measuring the decrease of NADPH oxidation at 340 nm. The reduction of DTNB was recorded as an increase in absorption at 412 nm (C and F).