TABLE 2.

Primers designed for this study

Gene	Strain(s) amplified	Primer targeta	Sequence (5′–3′)	Expected product size (bp)
Glyceraldehyde phosphate dehydrogenase	C7184 and JY1305	gapdh F	TGAAGGCGGTAACCTAATCG	97
		gapdh R	TACGTCAACACCGATTGCAT
Type IV pilin protein subunit	JY1305 only	pilA F	TCATTGGTGTGTTAGCCGCA	73
		pilA R	GCTGAGGCAGCTTCTGACTT
Type IV pilin protein subunit	C7184 only	pilA F	GCACAGCTCCAACCAGTAGT	57
		pilA R	TTGGCGGCACTTCAACAATG
Type IV pilin protein prepilin peptidase	JY1305 only	pilD F	GGCTTACTGGTAGGCAGCTT	126
		pilD R	GGTTTCTGTCGGCGGTGATA
Type IV pilin protein prepilin peptidase	C7184 only	pilD F	TTGGCTTACTGGTAGGCAGC	128
		pilD R	GGTTTCTGTCGGTGGTGTGA
N-Acetylglucosamine binding protein	C7184 and JY1305	gpbA F	TTGAGTGGACCTTTACCGCC	91
		gpbA R	CGGGCAAGTGGTTGATTTGG
Mannitol-sensitive hemagglutinin	C7184 and JY1305	mshA F	CAAGGCGGTTTCACCCTGAT	90
		mshA R	CAGATTTAGAAAACGCGGAGCC

^aF, forward; R, reverse.