TABLE 2.

Common genes from Venn diagram analysis of HT-29 cells cochallenged with poly(I·C) only versus poly(I·C) plus PC in descending order of Δ fold change of attenuation^a

Immune gene protein	Function	Immune pathway(s)	Fold change on challenge withb,c:		Δ fold change of attenuationc,d
			Poly(I·C) only	Poly(I·C) + PC
TNFAIP3	Component of ubiquitin-editing protein complex that inhibits NF-κB activation	NF-κB signaling, apoptosis	9.80 ↑	2.35 ↑	4.17 ↓
CXCL10 (chemokine)	Protein with pleiotropic effects, including stimulation of monocytes and natural killer cells and T cell migration	Cytokine-cytokine receptor interaction, chemokine signaling pathway, TLR signaling pathway	7.41 ↑	2.17 ↑	3.42 ↓
CXCL2 (chemokine)	Protein expressed at sites of inflammation	Cytokine-cytokine receptor interaction, chemokine signaling pathway	11.02 ↑	3.46 ↑	3.18 ↓
CXCL3 (chemokine)	Protein with chemotactic activity for neutrophils	Cytokine-cytokine receptor interaction, chemokine signaling pathway	12.36 ↑	3.90 ↑	3.17 ↓
BCL3	Contributor to the regulation of transcriptional activation of NF-κB pathway	NF-κB signaling, apoptosis	10.29 ↑	3.33 ↑	3.09 ↓
CX3CL1 (chemokine)	Protein with chemotactic activity for T cells and monocytes	Cytokine-cytokine receptor interaction, chemokine signaling pathway	7.20 ↑	2.68 ↑	2.68 ↓
CCL20 (chemokine)	Chemotactic factor that attracts lymphocytes and neutrophils	Cytokine-cytokine receptor interaction, chemokine signaling pathway	10.55 ↑	4.07 ↑	2.59 ↓
CXCL1 (chemokine)	Protein with chemotactic activity for neutrophils	Cytokine-cytokine receptor interaction, chemokine signaling pathway	16.31 ↑	6.77 ↑	2.41 ↓
BIRC3	Inhibitor of apoptosis by binding to TNF receptor-associated factors TRAF1 and TRAF2 (apoptotic suppressor)	Apoptosis	6.08 ↑	2.72 ↑	2.24 ↓
NFKBIE	Inhibitor of NF-κB by complexing with and trapping it in the cytoplasm	NF-κB signaling	5.28 ↑	2.63 ↑	2.00 ↓
LIF (cytokine)	Inducer of terminal differentiation in leukemic cells	Cytokine-cytokine receptor interaction, JAK-STAT signaling pathway	3.17 ↑	2.09 ↑	1.52 ↓
NFKBIA	Inhibitor of NF-κB/REL complexes by trapping REL dimers in the cytoplasm	NF-κB signaling	5.35 ↑	3.90 ↑	1.37 ↓
IL-8 (chemokine)	Potent chemotactic factor that attracts neutrophils, basophils, and T cells	Cytokine-cytokine receptor interaction, chemokine signaling pathway, TLR signaling pathway	12.98 ↑	9.79 ↑	1.33 ↓

^aImmune gene function data are from GeneCards (http://www.genecards.org), and immune pathway data are from KEGG (http://www.genome.jp/kegg/pathway.html).

^bFold change or expression ratio = 2^log₂ratio. ↑, upregulation.

^cA 2-fold change in differentially expressed genes and a P value of <0.05 were considered statistically significant.

^dΔ fold change = expression with poly(I·C)/[expression with poly(I·C) + expression with PC]. ↓, a reduction in the Δ fold change of HT-29 cells challenged with poly(I·C).