FIG 5.

ExpA impacts motility in PGA supplemented medium. Photographs show results from bacterial swimming motility plate assays. Plates were made from 0.3% (wt/vol) agar in M9 minimal medium supplemented with 0.2% PGA as the carbon source. Bacteria from the P. wasabiae wild type (WT), the expA mutant (ExpA), the expA mutant with complementation vector (ExpA + pBSexpA), and the vector control (ExpA + pBS) were grown overnight and subsequently pipetted onto the center of the plates. Plates were incubated for 2 days at 28°C, with at least five technical replicate plates per strain, and the experiment was repeated at least 3 times. Five-pointed asterisks indicate P values of 0.01 or less for comparison of the mean diameter of one strain to the other strains, using one-way ANOVA with the Scheffe post hoc test. However, diameters of the ExpA and ExpA+pBS vector control strains were not significantly different from each other (P < 0.01), as indicated by six-pointed asterisks.