FIG 3.

Multiple sequence alignment of Cb-ACR and other characterized close homologs. Gene identifiers of the genes can be found in Table 1. The sequence of the ketose reductase of Bemisia argentifolii (GI:4106363) was included because a three-dimensional structure was available (PDB 1E3J), which was used for the modeling of Cb-ACR. The sequences were aligned using the web-based T-Coffee program (22). Conserved residues involved in metal and NAD(P) binding are shaded in black. Asterisks indicate residues involved in binding of the catalytic and structural zinc. The secondary structure prediction of Cb-ACR (using the psipred program [59]) is shown above the alignment.