FIG 3.

Stimulation index of CD8⁺ CFSE^low T cells stimulated with F4 peptide pools (A and B) and frequency of F4- and TRAP-specific CD8⁺ CFSE^low T cells expressing at least one cytokine following primary and booster doses of the F4/AS01_B investigational vaccine (C and D) in the chloroquine (A and C) and the control (B and D) groups (ATP immunogenicity cohort). CFSE, 5,6-carboxyfluorescein diacetate succinimidyl ester; F4, pool of peptides covering the sequence of F4 antigens; PRE, prevaccination in the initial study; PII(D44), 44 days after primary vaccination; PII(D360), 360 days after primary vaccination; PREIII, prebooster dose given approximately 3 years post-primary vaccination; PIII(D14), 14 days after the booster dose; PIII(D180), 180 days after the booster dose; SI, stimulation index; TRAP, tartrate-resistant acid phosphatase (irrelevant peptides). In the box plot, the central box shows the interquartile range (Q1 to Q3), with the thick horizontal line representing the median (Q2) and the whiskers (above and below the box) representing the maximum and the minimum, respectively.