FIG 2.

Construction of pORF deletion mutants. (A) Each of the individual eight ORFs from the shuttle vector pGFP::CM was deleted with the deletion region indicated. (B) The deletion mutants designated pGFP::CMΔPgp1 to pGFP::CMΔPgp8, together with the parent plasmid (pGFP::CM), as listed on top of the gel image were detected for the presence of each of the 8 pORFs (from top to bottom rows) by PCR. (C) The eight plasmids with each deleted of one pORF were transformed into plasmid-free (pf) CMUT3 organisms. However, stable CMUT3 transformants were only obtained with four of the eight plasmids, and they were CMUT3 transformed with pGFP::CMΔPgp3, pGFP::CMΔPgp4, pGFP::CMΔPgp5, and pGFP::CMΔPgp7.