FIG 4.
ChiA is a soluble extracellular protein. (A and B) Spent media and whole cells of the wild type (WT), chiA mutant (chiA), and chiA mutant complemented with pSSK05 (chiAc) were examined for ChiA by SDS-PAGE followed by Western blotting. (C) Spent medium from a culture of wild-type F. johnsoniae UW101 was examined for ChiA before (spent medium) and after fractionation into soluble and insoluble (membrane) fractions by centrifugation at 352,900 × g for 30 min. For each panel, equal amounts of each sample based on the starting material were loaded in each lane. For cells this corresponded to 15 μg protein, whereas for spent medium this corresponded to the equivalent volume of spent medium that contained 15 μg cell protein before the cells were removed.