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. 2014 Mar;196(5):961–970. doi: 10.1128/JB.01170-13

FIG 6.

FIG 6

Mutations in T9SS genes disrupt secretion of ChiA. Cell-free spent media (A) and cells (B) were examined for ChiA by SDS-PAGE followed by Western blotting using antiserum against recombinant ChiA. WT, wild-type F. johnsoniae UW101. chiA, chiA mutant CJ1808. gldK, gldK mutant UW102-57. gldKc, UW102-57 complemented with pTB99, which carries gldK. gldL, gldL mutant UW102-344. gldLc, UW102-344 complemented with pTB81a, which carries gldL. gldM, gldM mutant UW102-176. gldMc, UW102-176 complemented with pTB94a, which carries gldM. ΔgldNO, gldNO deletion mutant CJ1631A. ΔgldNOc, CJ1631A complemented with pTB79, which carries gldN. sprA, sprA mutant UW102-3. sprAc, UW102-3 complemented with pSN48, which carries sprA. sprE, sprE mutant FJ149. sprEc, FJ149 complemented with pNap2, which carries sprE. sprT, sprT mutant KDF001. sprTc, KDF001 complemented with pKF002, which carries sprT. Samples loaded in panel B corresponded to 15 μg protein per lane, and samples loaded in panel A corresponded to the volume of spent medium that contained 15 μg cell protein before the cells were removed.