TABLE 2.

Enzymatic Identification of the PMD reaction product^a

Parameter	Value for sample:
	A	B	C	D	E
Selectivity of PMD product metabolism by IPK or MDD
(R)-MVA 5-P present in reaction mixture (nmol)	51.1 ± 2.5	55.2 ± 0.1
Product formation:
PMD + IPKb added to A (nmol)	52.2 ± 1.8
Product formation:
PMD + MDDc added to B (nmol)		2.0 ± 0.1
Reaction stoichiometry
(R)-MVA 5-P added (nmol)			25	50	75
HvPMD reaction product formed (nmol)			24.6 ± 2.5	51.1 ± 3.6	74.9 ± 0.3
PMD reaction product utilized by HvIPK (nmol)			24.7 ± 1.1	52.2 ± 1.8	76.5 ± 2.0
Reaction stoichiometry			1.01 ± 0.04	1.02 ± 0.01	1.02 ± 0.03

^aConversion of mevalonate 5-phosphate by PMD and subsequent ATP-dependent conversion of the PMD reaction product (or any unreacted mevalonate 5-phosphate) by secondary enzymes (IPK or MDD) was measured using the pyruvate kinase/lactate dehydrogenase-coupled assay described in Materials and Methods.

^bH. volcanii IPK (5 μg) was incubated with the PMD reaction product.

^cS. epidermidis MDD (20 μg) was incubated with the PMD reaction product.