TABLE 1.

Presence and absence of genomic regions across the genus Brucella and close relatives

Region	Other identifier	Presencea in:
		B. abortus	B. melitensis	B. ovis	Brucella sp. strain NVSL	B. ceti A	B. pinnipedialis	B. ceti B	B. canis-B. suis	B. suis bv. 5	B. neotomae	B. microti	Brucella sp. strain N8	Brucella sp. strain BO1	Brucella sp. strain BO2	O. anthropi	O. intermedium	B. quintana	M. loti	A. tumefaciens
1	GIBs2	✓	✓b	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	0	0	0	0	0
2		✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	0	0	0	0	0
3		✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	0	0	0	0	0
4	SAR 1–14	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	0	0	0	0	0
5		✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	0	0	0	0	0
6	mgtC	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	0	0	0	0	0
7		✓	✓	✓	✓	✓c	✓	✓	✓	✓	✓	✓	✓	✓	✓	0	0	0	0	0
8		✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	0	0	0	0	0
9		✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	0	0	0	0	0
10		✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	0	0	0	0	0
11	SAR 2–7	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	0k	✓	✓	0	0	0	0	0
12	SAR 2–8	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	0	0	0	0	0
13		✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	0l	0	0	0	0
14		✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	0	✓	✓d	0	0	0	0	0
15	virB	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	#	#	#	#	#
16	wbk	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	0m	0n	✓e	0	0	0
Classic 1	SAR 1–2	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	0	0	0	0	0	0	0	0
Classic 2	SAR 1–5	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	0	0	0	0	0	0	0	0
Classic 3	SAR 1–6	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	0	0	0	0	0	0	0	0
Classic 4	GI2	✓	✓	0o	✓	✓	✓	✓	✓	✓	✓	✓	0	0p	0	0	0	0	0	0
Classic 5	GI1	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	0	0	0	0	0	0	0	0
Classic 6	SAR 1–12	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	0	0	0	0	0	0	0	0
Classic 7	SAR 1–16	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	0	0	0	0	0	0	0	0
Classic 8	SAR 1–17	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓f	0	0	0	0	0	0	0
Classic 9	IncP	✓g	0	0	✓	✓	✓	✓	✓	0	✓	✓	0	0	0	0	0	0	0	0
Classic 10	SAR 2–10	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	✓	0	0	0	0	0	0	0	0
Classic 11	GI5	✓	✓	0	✓h	✓	✓	✓	✓	✓	✓	✓	0	0	0	0	0	0	0	0
Classic 12	GI3	✓	✓	✓	0	0	✓i	✓	0q	✓	✓	0	0	0	0	0	0	0	0	0
Classic 13	26.5 kb	0	0	✓	0	✓j	0	0	0	0	0	0	0	0	0	0	0	0	0

^a✓, region is present; 0, region is absent; #, genes of this type are present but are not homologs to those found in Brucella.

^bBrucella melitensis 16M, 16M WGS, and Rev1 are missing 5 of the 9 genes in this region.

^cBrucella sp. strain F5/99 is missing all genes in this region.

^dBrucella inopinata BO2 is missing 5 of the total 24 genes.

^eOf the 12 genes in this area important for forming the LPS in Brucella, Ochrobactrum intermedium has 7.

^fBrucella sp. strains 83/13 and NF2653 have all genes in this region.

^gBrucella abortus bv. 5 strain B3196 has 9 of the 16 genes in this region.

^hBrucella sp. strain NVSL has 22 of the 37 genes in this region.

ⁱPresent in both Brucella pinnipedialis B2/94 and M163/99/10, but all 27 genes are missing in M292/94/1.

^jAll genes missing Brucella ceti M490/95/1 and B1/94, but all are present in B. ceti Cudo and Brucella sp. strain F5/99. Similarly, all genes are missing in B. pinnipedialis M163/99/10, and all present in strains B2/94 and M292/94/1. B. ceti A includes B. ceti strains Cudo, F5/99, M490/95/1, and B1/94, while B. ceti B includes strains M13/05/1 and M644/93/1.

^kBrucella sp. strains 83/13 and NF2653 have 2 of the 8 genes in this region.

^lOchrobactrum anthropi has only 2 of the 11 genes in this region.

^mBrucella inopinata BO2 has only 2 of the 12 genes in this region that other brucellae have. It has four genes in the rml operon in the same place.

ⁿOchrobactrum anthropi has only 2 of the 12 genes in this region that other brucellae have. It has four genes in the rml operon in the same place.

^oBrucella ovis is missing 21 of the 22 genes in this region.

^pBrucella inopinata BO1 has only 5 of the 22 genes in this region.

^qBrucella suis Thomsen (ATCC 23445) is the only member of this clade that has any of these genes, and it has all 28 of them.