FIG 2.

Growth with mannopine induces production of AAI and conjugative transfer of pAoF64/95. Strains F64/95 (A) and NTL6(pAoF64/95) (B) were grown in AB medium with 0.005% yeast extract and mannitol (open triangles and open bars) or MOP (open circles and gray bars). Growth was followed turbidometrically using a Klett colorimeter. At approximately 8-h intervals, a portion of each culture was removed and assayed for conjugative-transfer efficiency (black circles) and accumulation of AAI (bars). The amount of extractable AAI in the culture supernatant is indicated over the corresponding bar. The transfer frequency is expressed as the number of transconjugates recovered per input donor cell.