FIG 3.

Physicogenetic map of pAoF64/95. The complete 176,574-bp sequence of pAoF64/95 was determined, and the sequence was annotated as described in Materials and Methods. Significant open reading frames are shown as boxes, with those on the outside oriented in a clockwise direction and those on the inside oriented in a counterclockwise direction. The genes are color coded with respect to known or putative functions as follows: yellow, replication; violet, uptake and catabolism of MOA; magenta, uptake and catabolism of AGA; cyan, uptake and catabolism of MOP; orange, uptake and catabolism of agrocinopines; blue, conjugative transfer; red, regulation of MOP catabolism or conjugative transfer; green, three putative integrase genes; lavender, a segment that is largely syntenic in gene composition and order with similarly sized regions of two Ri plasmids, pRi1724 and pRi2659; gray, other genes that have a returned hit in the NCBI nonredundant sequence database; black, ORFs with no significant similarities to genes in the databases. The extents of the inserts in each cosmid in the ordered library are represented by the arcs within the rings. The annotated sequence is available in GenBank (see the text).