FIG 9.

TraM inhibits TraR-dependent induction of AAI production and conjugative transfer. (A and B) The traM mutant, NTL4(pAoF64/95::traMpVik107), was assessed for conjugative transfer (A) and AAI accumulation (B) when grown on AB medium supplemented with either mannitol or MOP. (C and D) The mutant was complemented in trans with a wild-type copy of traM cloned into pSRKGm. This strain, NTL4(pAoF64/95::traMpVik107, pSRKGm::traM), was continuously cultured in AB minimal medium supplemented with either mannitol or MOP and with or without IPTG to induce expression of traM, as described in Materials and Methods. Samples were removed after the indicated numbers of cumulative generations, and the cells were tested for conjugative competence (C) and AAI accumulation (D), as described in Materials and Methods. The numbers of generations are approximate. Each experiment was done two or three times, and the means and standard deviations are shown.