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SDS-PAGE analysis of recombinant MG_427 protein. Purified recombinant MG_427 protein was treated with DTT or peroxides for 1 h at room temperature. Proteins were resolved by nonreducing 12% SDS-PAGE and visualized by Coomassie blue staining. PL, protein ladder. a and b, the lower and upper band, respectively; c, the band corresponding to dimers of MG_427.
