FIG 3.

Generation of ΔMG_427 mutant and complementation strain. (A) Schematic representation of the strategy for generating the MG_427 deletion mutant by homologous recombination. Downward-pointing arrows represent HindIII cleavage sites. Lines underneath indicate fragments that distinguish the wild type from the MG_427 deletion mutant. (B) Southern hybridization profiles. WT, wild type; ΔMG_427, deletion mutant. (C) Western blot. Whole-cell lysates from the WT, ΔMG_427, and complemented mutant strains were resolved by SDS-PAGE, and proteins were transferred to a nitrocellulose membrane. The membrane was probed with polyclonal antibodies against the adhesin P140 (MG_191) or MG_427.