FIG 5.

Subcellular location of MG_427 in M. genitalium. Equal amounts (5 μg/well) of whole-cell lysate (W), cytoplasmic (C), and membrane (M) fractions of M. genitalium were resolved by 4 to 12% NuPAGE and transferred to a nitrocellulose membrane. The membrane was cut into three pieces on the basis of the expected protein sizes before exposure to specific antibodies (anti-P140, anti-EF-G, and anti-rMG_427). Adhesin P140 (MG_191) served as the positive control for membrane protein, while EF-G, a cytoplasmic protein, served as the internal cytosolic control.