FIG 6.

Transcription of MG_427 under various stress conditions and identification of the putative promoter. (A) Cultures of M. genitalium were grown in SP-4 broth to logarithmic phase and exposed to various stresses (indicated on the top) for 1 h before total RNA was isolated. Northern blot analysis was performed as described in Materials and Methods. (B) Relative levels of MG_427 determined by densitometry. (C) Putative promoter region of MG_427. The putative −10 region and −35 region for σ⁷⁰ are bold-faced and underlined. The transcriptional start site (TSS) is shown in brackets. The sequence from MG_426 is italicized.