TABLE 1.
Primers used in this study
| Primer name | Sequence (5′ to 3′)a |
|---|---|
| 5′MG_427 (NdeI) | GGAATTCCATATGGATAAAAAATACGATATCACAG |
| 3′MG_427 (XhoI) | CCGCTCGAGTTAGTGGACTAAAGTAACGCTAA |
| Overlap F | ACATTCAC(TGG)GAAATTCACTC |
| Overlap R | GAGTGAATTTC(CCA)GTGAATGT |
| 5′UMG_427 (BamHI) | CGCGGATCCTGGGATCTTTATTGGATCAC |
| 3′UMG_427 (NotI) | TAAGAATGCGGCCGCATATGCTTATGGATTAAGTTTAACTTAAAT |
| 5′DMG_427 (XhoI) | CCGCTCGAGTAGCGTTACTTTAGTCCACTAAAAC |
| 3′DMG_427 (XbaI) | GCTCTAGAGATAGGATGAATAAAAACGTTAGTTATC |
| 5′MG_427C (NotI) | ATAAGAATGCGGCCGCACTAATGGTAAAGTAACACGGATTC |
| 3′MG_427C (BamHI) | CGCGGATCCTTAGTGGACTAAAGTAACGCTAAT |
| MG_427SP1 | CCACGTTTACTTACAAAATCAATG |
| MG_427SP2 | GAGTGAATTTCTCAGTGAATGT |
a
Underlined sequences are the restriction enzyme recognition sequences. The codon change from TGA to TGG is included in parentheses.