FIG 1.

Repression of rpoS by c-di-GMP requires VpsR and VpsT. Strain HX11, containing a chromosomally integrated rpoS-lacZ fusion (wild type [WT]), and isogenic mutant strains HX15 (ΔvpsR), HX19 (ΔvpsT), HX21 (ΔvpsR ΔvpsT), HX12 (ΔhapR), HX16 (ΔhapR ΔvpsR), and HX20 (ΔhapR ΔvpsT) were transformed with plasmid pAT1662 expressing the DGC VCA0956. Expression of VCA095 was induced as described in Materials and Methods, and β-galactosidase activity was measured as an indicator of rpoS expression. Each value represents the mean for six independent cultures, and error bars indicate standard deviations (*, significantly different from uninduced cultures [P < 0.01]). Open bars, uninduced cultures; filled bars, induced cultures.