FIG 5.

Binding of DctB-His (DctB-H) and DctA-His (DctA-H) on DctS-Strep (DctS-S) and copurification by mSPINE. The three proteins were coexpressed in E. coli C43(DE3). DctS-S was isolated from the solubilized membrane fraction using a Strep-Tactin column. The proteins were detected in Western blots by antibodies directed against the His₆ or Strep tag, respectively. After blotting of the proteins, the nitrocellulose membrane was divided and the antibodies were used separately for detection of proteins. As a control, DctA-H and DctB-H were expressed without DctS-S and the membrane fraction was applied to a Strep-Tactin column.