TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Relevant characteristicsa | Source or reference |
|---|---|---|
| Strains | ||
| E. coli TOP10 | F− mcrA Δ(mrr-hsdRMS-mcrBC) ϕ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara-leu)7697 galU galK rpsL (Strr) endA1 nupG | Invitrogen |
| R. solanacearum | ||
| UW551 (WT) | Wild-type race 3, biovar 2 | 8 |
| UW551Rif (WTRif) | UW551; Rifr | 25 |
| epsB mutant | UW551 ΔepsB::Km; Kmr | 14 |
| nasA mutant | UW551 ΔnasA::Gm; Gmr | This study |
| nasA mutant + pNascomp | UW551 ΔnasA::Gm + pNascomp; Gmr Kmr | This study |
| nasA mutant + pUFJ10 | UW551 ΔnasA::Gm + pUFJ10; Gmr Kmr | This study |
| Plasmids | ||
| pSTBlue-1 | Cloning vector; Ampr Kmr | EMD Bioscience |
| pCR-Blunt | Cloning vector; Kmr | Invitrogen |
| pUFJ10 | Gmr Kmr; stable in R. solanacearum | 23 |
| pSTBlueΔnasA | pSTBlue-1 + nasA::Gm deletion construct in EcoRV MCS | This study |
| pNascomp | pUFJ10 + narK3 nirB-nirD-nasB-nasA DNA subcloned from pCR-Blunt | This study |
a
Gmr, gentamicin resistance; Rifr, rifampin resistance; Ampr, ampicillin resistance; Strr, streptomycin resistance; Kmr, kanamycin resistance.