TABLE 3.

Expression of R. solanacearum genes involved in nitrate assimilation and scavenging during tomato pathogenesis^a

Gene	Annotation (NCBI)	R. solanacearum UW551		R. solanacearum GMI1000
		Locus tag	In planta absolute expression	Locus tag	In planta absolute expression
narK3	High-affinity nitrate transporter transmembrane protein	RRSL_01120	9.66	RSp1223	8.95
nirB	Nitrite reductase NADPH large subunit	RRSL_01119	9.72	RSp1222	8.94
nirD	Nitrite reductase NADPH small subunit	RRSL_01118	7.97	RSp1221	8.29
nasB	Flavoprotein FAD oxidoreductase	RRSL_01117	8.26	RSp1220	7.94
nasA	Nitrate reductase large subunit oxidoreductase	RRSL_01116	7.08	RSp1219	7.95
nasF	Nitrate transporter protein	RRSL_03280	9.01	RSc0381	9.83
nasE	Nitrate transmembrane ABC transporter protein	RRSL_03281	7.80	RSc0380	9.17
nasD	Nitrate binding ABC transporter protein	RRSL_03282	8.71	RSc0379	7.97
glnA1	Glutamine synthetase	RRSL_01421	11.71	RSc1258	14.03
ntrB	Nitrogen regulation (sensor protein kinase transcription regulator)	RRSL_01419	9.68	RSc1260	11.31
ntrC	Nitrogen assimilation regulatory response regulator transcription regulator protein	RRSL_01418	9.84	RSc1261	9.95
glnA2	Glutamine synthetase	RRSL_03114	8.51	RSp0886	8.41
glnB	Nitrogen regulatory P-II transcription regulator protein	RRSL_00967	12.05	RSc2345	12.91
amtB	Ammonium transporter	RRSL_03319	10.07	RSc0343	10.88
glnK	Nitrogen regulatory P-II transcription regulator protein	RRSL_03320	10.68	RSc0342	11.51

^aTo determine the in planta absolute expression, R. solanacearum cells were centrifuged from the stems of symptomatic tomato plants inoculated with the indicated strain, and absolute gene expression levels were determined using strain-specific microarrays as described previously (4).