TABLE 1.
Temperature stability of purified wild-type and DadD mutant strains
| DadD | Temp (°C) | % of retained activitya |
|---|---|---|
| WT | 60 | 90 |
| 70 | 34 | |
| Y136R | 50 | 80 |
| 60 | 40 | |
| E150R | 50 | 96 |
| 60 | 25 | |
| Y136R & E150R double mutant | 25 | 100 |
| 50 | 35 |
That is, after heating for 10 min at the indicated temperature.