TABLE 1.
Plasmids and strains used in the study
| Plasmid or strain | Relevant genotype and/or characteristic(s) | Source or reference |
|---|---|---|
| Plasmids | ||
| pTS1 | Shuttle vector; pE194ori(Ts); ColE1 bla cat | 74 |
| pNL9164 | Shuttle vector; pT181 cop-634(Ts) repC4 ori | 75 |
| pKM1 | S. aureus codY cloned into pBAD30 with 5 additional histidine codons at the C terminus | K. McCarty and A. Sonenshein, unpublished data |
| pCL15 | Expression shuttle vector, derivative of pSI-1; PSPAC cat | 44, 46 |
| pAR12 | DNA regions surrounding agrA cloned in pST1 | This study |
| pAR23 | DNA regions surrounding the P2-P3 agr promoters cloned in pST1 | This study |
| pAR28 | Cloned intron DNA targeted to SACOL2585 in pNL9164 | This study |
| pAR31 | DNA regions surrounding agrC cloned into pST1 | This study |
| pTL6936 | pCL15 PSPAC-codY | 26 |
| PSPAC-agrA | pCL15 PSPAC-agrA | This study |
| S. aureus strains | ||
| UAMS-1 | Clinical isolate | 76 |
| MS1 | UAMS-1 ΔcodY::ermC | 26 |
| AR87 | UAMS-1 SA2585::intron | This study |
| AR89 | UAMS-1 ΔcodY::ermC SA2585::intron | This study |
| CM18 | UAMS-1 agr::tetM | 25 |
| CM20 | UAMS-1 sarA::kan | 25 |
| CM22 | UAMS-1 ΔcodY::ermC sarA::kan | 25 |
| AR45 | UAMS-1 ΔagrA | This study |
| AR48 | UAMS-1 ΔcodY::ermC ΔagrA | This study |
| AR70 | UAMS-1 ΔP2-P3 | This study |
| AR71 | UAMS-1 ΔcodY::ermC ΔP2-P3 | This study |
| AR145 | UAMS-1 ΔagrC | This study |
| AR147 | UAMS-1 ΔcodY::ermC ΔagrC | This study |