TABLE 4.

CodY does not regulate the expression of agrA through binding within agrC^a

Strain	Level of mRNAb
	agrB	agrA
WT (UAMS-1)	17.17 ± 0.49	18.15 ± 2.19
UAMS-1 ΔP2-P3	ND	0.52 ± 0.02
UAMS-1 ΔcodY ΔP2-P3	ND	0.51 ± 0.01

^aExponential-phase culture samples (OD₆₀₀, between 0.5 and 0.8) of UAMS-1, UAMS-1 ΔP2-P3, and UAMS-1 ΔcodY ΔP2-P3 were collected, and RNAs were prepared. The absolute levels of agrB and agrA mRNAs were determined by real-time quantitative PCR.

^bExpressed as the number of copies of agr mRNA per 10⁶ copies of 16S rRNA. ND, not detectable.