FIG 5.

In trans complementation of the Δsap mutant with B. anthracis and B. cereus sap genes. The recombinant plasmids carrying the sap genes from B. anthracis Sterne and B. cereus strains RS438 and RS756 (E33L) were transformed into BA750 (Δsap), and the resulting transformants were tested for AP50c sensitivity by spot tests. Ten microliters of 10× serial dilutions of phage was spotted on a lawn of each strain. Clearing in the spots after overnight incubation indicates lysis of the bacteria and phage sensitivity. (A) 7702; (B) BA750 (Δsap); (C) BA750 complemented with RS438 sap; (D) BA750 complemented with RS756 (E33L) sap; (E) BA750 complemented with 34F₂ sap. In panel C, undiluted AP50c phage stock was spotted both in the center of the plate and at the 12 o'clock position; in the other panels, undiluted phage stock was spotted only at the 12 o'clock position.