TABLE 4.
Amino acid differences in select positions among selected B. anthracis, B. cereus, and B. thuringiensis Sap proteins
| Strain | Amino acid at positiona: |
AP50c adsorptionb | AP50c plating | ||||||
|---|---|---|---|---|---|---|---|---|---|
| 57 | 190 | 405 | 421 | 571 | 586 | 654 | |||
| B. anthracis Ames | K | T | S | V | T | T | K | +++ | Yes |
| B. thuringiensis serovar pulsiensis BGSC 4CC1 | K | T | S | V | T | T | K | +++ | No |
| B. cereus RS438 CDC2000032805 | K | T | A | V | K | T | E | +++ | Yes |
| B. cereus RS756 (E33L ZK) | T | S | S | V | K | T | K | +++ | Yes |
| B. thuringiensis serovar monterrey BGSC 4AJ1 | K | T | S | L | T | T | K | ++ | No |
| B. thuringiensis serovar pondicheriensis BGSC 4BA1 | K | T | S | L | T | K | K | − | No |
| B. cytotoxicus NVH 391–98c | K | S | S | V | T | K | K | NT | NT |
| B. cereus 03BB102c | E | S | A | L | T | T | K | NT | NT |
| B. cereus AH187c | A | S | V | L | K | E | A | NT | NT |
| B. cereus NC7401c | A | S | V | L | K | E | A | NT | NT |
| B. cereus FRI-35c | A | S | V | L | K | N | A | NT | NT |
| B. cereus Q1c | A | S | V | L | K | K | D | NT | NT |
| B. cereus biovar anthracis CIc | E | S | V | L | K | E | A | NT | NT |
| B. cereus AH820c | E | S | V | L | K | E | A | NT | NT |
| B. cereus F837/76c | E | S | V | L | K | E | A | NT | NT |
| B. thuringiensis Al Hakamc | E | S | V | L | K | E | A | NT | NT |
a
Position of the amino acid residue in the 814-amino-acid Sap protein of B. anthracis Ames; residues in bold are those that differ from the residues of Sap of B. anthracis Ames.
b
+++, >66 to 100% adsorption; ++, >34 to 66% adsorption; −, 0% adsorption; NT, not tested.
c
Additional differences in the Sap proteins of these strains are not listed.