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[2-14C]DHU radiolabeling of the wild-type (BW25113) and isc mutant strains that lack the genes essential for Fe-S cluster biosynthesis. Radioactivity (14C cpm) of the wild-type strain (BW25113) and the isc mutant strains, which were aerobically grown in 5 ml of LB medium containing 0.5 μM [2-14C]DHU (962 Bq · ml−1) to stationary phase, was measured with a liquid scintillation counter. The means ± standard deviation of three independent measurements is shown.
