FIG 4.

Fe-S enzyme activity of the knockouts identified by the radiolabeling screen under microaerobic or anaerobic conditions. The crude extracts were prepared from the strains cultivated under a microaerobic (A) or an aerobic (B) conditions. The specific activities (S.A.) of the Fe-S enzymes, (a) dihydropyrimidine dehydrogenase (DPD), (b) 6-phosphogluconate dehydratase (6-PG), and (c) glutamate synthase (GS) from the wild-type and mutant strains were determined. The activity of glucose 6-phosphate dehydrogenase (G6PDH), which does not contain an Fe-S cluster, was measured as a control (d). The means ± standard errors from two independent measurements are shown.