FIG 3.

Transcript levels of QS-controlled genes in chemostat and batch cultures. Relative abundances of pepB (a), lasB (b), phzA1 (c), and rhlA (d) mRNAs were measured by qPCR. Wild-type P. aeruginosa was cultured in glutamate minimal medium in a P- or C-limited chemostat (left) and in batch culture (right). (Left) A lasR mutant, an rhlR mutant, and a lasR rhlR double mutant (indicated as “both”) were cultured in a P-limited chemostat for comparison with the wild type. Chemostat cultures were at a steady-state OD₆₀₀ of 0.15. (Right) Batch cultures were sampled during the exponential (E), early-stationary (ES), and late-stationary (LS) phases, at the indicated densities. Values are means for three independent biological replicates. Error bars indicate standard deviations of the means.