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Kinetic parameters of TK1814a
| Substrate | Mean Vmax (μmol min−1 mg−1) ± SD | Mean Km (mM) ± SD | Mean kcat (s−1) ± SD | kcat/Km (mM−1 s−1) |
|---|---|---|---|---|
| Aspartate | 1.13 ± 0.04 | 0.47 ± 0.04 | 0.80 ± 0.03 | 1.70 |
| Glutamate | 0.26 ± 0.05 | 1.92 ± 0.50 | 0.18 ± 0.04 | 0.09 |
ADC and GAD activities with various concentrations of Asp or Glu were measured in the presence of 0.25 mM PLP. Values are means ± standard deviations or ratios.
