TABLE 2.
Evaluation of module 2 (aminoglycosides) of the AID TB Resistance assay using clinical M. tuberculosis strains (Switzerland, n = 4; South Africa, n = 18) and in vitro-selected M. bovis BCG mutant strains (n = 5)
| No. of isolates from: |
Streptomycina |
Amikacin-capreomycin, rrsb |
|||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| RpsL |
rrs |
||||||||||||||||||
| AID module 2 |
DNA sequencing | AID module 2 |
DNA sequencing | AID module 2 |
DNA sequencing | ||||||||||||||
| Switzerland | South Africa | wt 43 | K43R | wt 88 | K88R | K88Q | wt 513–517 | C513T | A514C | G515C | C517T | wt 1401/1402 | A1401G | C1402T | wt 1484 | G1484C/T | |||
| 2 | 3 | − | + | + | − | − | K43R | + | − | − | − | − | ND | − | + | − | + | − | A1401G |
| 2 | − | + | + | − | − | K43R | + | − | − | − | − | wt | + | − | − | + | − | wt | |
| 3b | − | + | + | − | − | K43R | + | − | − | − | − | ND | − | − | + | + | − | C1402T | |
| 2b | − | + | + | − | − | K43R | + | − | − | − | − | ND | + | − | − | − | + | G1484T | |
| 1 | + | − | − | + | − | K88R | + | − | − | − | − | ND | + | − | − | + | − | ND | |
| 4 | + | − | + | − | − | ND | − | − | + | − | − | A514C | − | + | − | + | − | A1401G | |
| 2 | + | − | + | − | − | wt | − | − | + | − | − | A514C | − | + | − | + | − | A1401G | |
| 1 | + | − | + | − | − | wt | − | − | + | − | − | A514C | + | − | − | + | − | wt | |
| 1 | + | − | + | − | − | wt | − | − | − | − | + | C517T | + | − | − | + | − | ND | |
| 6 | + | − | + | − | − | wt | + | − | − | − | − | wt | + | − | − | + | − | wt | |
a
−, no signal (band); +, signal (band); wt, wild-type; ND, not determined.
b
Mycobacterium bovis BCG 1721 (RpsL K43R) derivatives selected for kanamycin-capreomycin resistance.