TABLE 7.
Evaluation of AID TB resistance line probe assay module 1 for detection of isoniazid and rifampin resistance in smear-positive clinical samples (n = 98)
| Smear result | No. of clinical samples | Isoniazida |
Rifampin, RpoB |
|||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
|
inhA |
KatG |
|||||||||||||
| AID module 1 |
DNA sequencing | AID module 1 |
DNA sequencing | AID module 1 |
DNA sequencing | |||||||||
| wt (−16, −15, −8) | Mutant (−16, −15, −8) | wt 315 | mut315 | wt 513–516 | mut516 | wt 522–526 | mut526 | wt 529–533 | mut531 | |||||
| Positive | 1b | − | + | C15T | − | − | wt | + | − | + | − | − | + | S531L |
| Positive | 1c | − | + | C15T | + | − | ND | + | − | + | − | + | − | ND |
| Positive | 6d | + | − | wt | − | + | S315T | + | − | + | − | + | − | ND |
| Positive | 1e | + | − | wt | − | + | S315T | − | − | + | − | + | − | L511P, D516G |
| Positive | 89f | + | − | ND | + | − | ND | + | − | + | − | + | − | ND |
a
In cases in which no mutations for isoniazid and/or rifampin were detected, wt signals were confirmed by DST only. ND, not done; −, no signal (band); +, signal (band); wt: wild-type.
b
Respiratory specimen (n = 1).
c
Respiratory specimen (n = 1).
d
Respiratory (n = 4), biopsy (n = 1), and abscess (n = 1) specimens.
e
Respiratory specimen (n = 1).
f
Respiratory (n = 78), tissue (n = 3), wound (n = 1), lymph node (n = 3), abscess (n = 2), biopsy (n = 1), and unspecified (n = 1) specimens.