TABLE 1.

Reference strains and clinical isolates used as controls for development of real-time PCR assays targeting the recN and gyrB genes

Group	Species	Straina	qPCR result
Streptococci	Streptococcus gallolyticus	ATCC 9809	recN SGG (+)
	Streptococcus gallolyticus subsp. gallolyticus	IMPG RS-51	recN SGG (+)
	Streptococcus infantarius subsp. coli	IMPG RS-52	gyrB SCI (+)
	Streptococcus infantarius subsp. infantarius	IMPG RS-54	gyrB SII (+)
	Streptococcus agalactiae	ATCC 13813	No amplification
	Streptococcus salivarius	ATCC 7073	No amplification
	Streptococcus mutans	ATCC 25175	No amplification
	Streptococcus oralis	ATCC 10557	No amplification
	Streptococcus parasanguinis	ATCC 903	No amplification
	Streptococcus pyogenes	ATCC 19615	No amplification
	Streptococcus pneumoniae	ATCC 33400	No amplification
Other bacteria	Enterococcus faecalis	ATCC 29212	No amplification
	Enterococcus faecium	ATCC 6569	No amplification
	Escherichia coli	ATCC 25922	No amplification
Clinical isolates	Streptococcus gallolyticus subsp. gallolyticus	HMV 317831	recN SGG (+)
	Streptococcus gallolyticus subsp. pasteurianus	HUSM 2011	recN SGP (+)
	Streptococcus gallolyticus subsp. pasteurianus	HMD 6075	recN SGP (+)
	Streptococcus gallolyticus subsp. gallolyticus	HMD 6925	recN SGG (+)
	Streptococcus gallolyticus subsp. gallolyticus	HMD 305	recN SGG (+)

^aSources of reference strains: INCQS, National Institute of Quality Health Control—Fiocruz; IMPG, Culture Collection of Paulo de Góes Microbiology Institute—UFRJ.