TABLE 1.
Characteristics of the 16 polymicrobial suspensions tested in the manual and automated inoculation and streaking
Suspension no. | No. of strains | Initial suspension concn (CFU/ml) fora: |
|||
---|---|---|---|---|---|
S. agalactiae | P. aeruginosa | S. aureus | E. coli | ||
1 | 2 | 105 | 105 | ||
2 | 2 | 103 | 105 | ||
3 | 2 | 107 | 105 | ||
4 | 2 | 104 | 107 | ||
5 | 3 | 105 | 105 | 105 | |
6 | 3 | 103 | 105 | 105 | |
7 | 3 | 105 | 103 | 107 | |
8 | 3 | 107 | 105 | 103 | |
9 | 3 | 104 | 105 | 106 | |
10 | 3 | 106 | 104 | 105 | |
11 | 3 | 105 | 106 | 105 | |
12 | 4 | 105 | 105 | 105 | 105 |
13 | 4 | 107 | 106 | 105 | 104 |
14 | 4 | 106 | 107 | 104 | 105 |
15 | 4 | 105 | 104 | 106 | 107 |
16 | 4 | 104 | 105 | 105 | 106 |
Suspensions were mixed volume to volume, i.e., 1:1, 1:1:1, and 1:1:1:1, to produce final suspensions of two, three, and four species, respectively.