TABLE 1.
Peptides identified in a MALDI-FT-ICR mass spectrum (Fig. 2) of a whole-virus digest of the A/Puerto Rico/8/34/Mount Sinai (H1N1) reference strain
| [M+H]+
m/z mono.a |
Difference (ppm) | Viral protein | Residues | Sequence | |
|---|---|---|---|---|---|
| Theoretical | Experimental | ||||
| 545.3411 | 545.3418 | −1.23 | HA | 416–419 | LEKR |
| 659.3153 | 659.3166 | −1.97 | HA | 158–162 | SSFYR |
| 676.3816 | 676.383 | −2.04 | HA | 330–335 | MVTGLR |
| 780.4296 | 780.431 | −1.78 | HA | 131–136 | FEIFPK |
| 801.4331 | 801.4347 | −1.95 | HA | 236–242 | VRDQAGR |
| 896.5569 | 896.5585 | −1.74 | HA | 63–71 | GIAPLQLGK |
| 904.4675 | 904.4692 | −1.91 | HA | 419–425 | RMENLNK |
| 907.4889 | 907.49 | −1.19 | HA | 412–418 | EFNKLEK |
| 1,016.5781 | 1,016.5806 | −2.49 | HA | 163–170 | NLLWLTEK |
| 1,125.554 | 1,125.5559 | −1.66 | HA | 505–513 | YSEESKLNR |
| 1,137.736 | 1,137.7385 | −2.23 | HA | 61–71 | LKGIAPLQLGK |
| 1,187.6425 | 1,187.6452 | −2.31 | HA | 226–235 | FTPEIAERPK |
| 1,343.7436 | 1,343.7471 | −2.63 | HA | 225–235 | RFTPEIAERPK |
| 1,443.6367 | 1,443.6398 | −2.14 | HA | 475–486 | EIGNGCFEFYHK |
| 1,626.8314 | 1,626.8356 | −2.58 | HA | 402–415 | MNIQFTAVGKEFNK |
| 1,677.8852 | 1,677.8889 | −2.21 | HA | 163–176 | NLLWLTEKEGSYPK |
| 2,633.2232 | 2,633.225 | −0.69 | HA | 203–224 | EQQNIYQNENAYVSVVTSNYNR |
| 604.3055 | 604.3066 | −1.87 | NA | 88–92 | DNSIR |
| 629.3047 | 629.3053 | −0.89 | NA | 333–337 | GFSYR |
| 793.3957 | 793.3975 | −2.3 | NA | 136–141 | DRSPYR |
| 1,021.5219 | 1,021.5237 | −1.72 | NA | 338–346 | YGNGVWIGR |
| 1,409.7177 | 1,409.7187 | −0.68 | NA | 81–92 | GWAIYSKDNSIR |
| 2,149.9289 | 2,149.9327 | −1.75 | NA | 354–371 | HGFEMIWDPNGWTETDSK |
| 2,639.1989 | 2,639.2005 | −0.6 | NA | 354–375 | HGFEMIWDPNGWTETDSKFSVR |
| 574.2949 | 574.2961 | −2.07 | NP | 200–204 | GINDR |
| 622.3102 | 622.3119 | −2.79 | NP | 205–208 | NFWR |
| 631.3779 | 631.3788 | −1.41 | NP | 442–446 | TEIIR |
| 661.3997 | 661.3999 | −0.29 | NP | 356–361 | GKLSTR |
| 672.4408 | 672.4417 | −1.27 | NP | 262–267 | SALILR |
| 676.3088 | 676.3105 | −2.45 | NP | 157–162 | TGMDPR |
| 708.3833 | 708.3846 | −1.8 | NP | 385–389 | YWAIR |
| 731.4052 | 731.4063 | −1.52 | NP | 176–184 | SGAAGAAVK |
| 761.3946 | 761.396 | −1.81 | NP | 417–422 | NLPFDR |
| 763.4103 | 763.412 | −2.27 | NP | 92–98 | TGGPIYR |
| 831.4325 | 831.4336 | −1.36 | NP | 20–26 | QNATEIR |
| 850.3915 | 850.3922 | −0.79 | NP | 237–243 | AMMDQVR |
| 887.5063 | 887.507 | −0.79 | NP | 175–184 | RSGAAGAAVK |
| 891.5052 | 891.5077 | −2.77 | NP | 91–98 | KTGGPIYR |
| 919.5114 | 919.5134 | −2.2 | NP | 92–99 | TGGPIYRR |
| 951.5165 | 951.5179 | −1.51 | NP | 383–389 | SRYWAIR |
| 961.4452 | 961.4468 | −1.71 | NP | 392–400 | SGGNTNQQR |
| 978.5373 | 978.5399 | −2.69 | NP | 228–236 | GKFQTAAQK |
| 1,023.4999 | 1,023.4996 | 0.27 | NP | 462–470 | GVFELSDEK |
| 1,135.5397 | 1,135.5421 | −2.09 | NP | 205–213 | NFWRGENGR |
| 1,177.5867 | 1,177.5888 | −1.81 | NP | 200–208 | GINDRNFWR |
| 1,186.6796 | 1,186.6824 | −2.38 | NP | 56–65 | LIQNSLTIER |
| 1,191.623 | 1,191.6253 | −1.94 | NP | 185–195 | GVGTMVMELVR |
| 1,207.6179 | 1,207.6206 | −2.25 | NP | 185–195 | GVGTMoxVMELVR |
| 1,221.6262 | 1,221.6283 | −1.75 | NP | 437–446 | TSDMRTEIIR |
| 1,223.6207 | 1,223.6224 | −1.41 | NP | 66–75 | MVLSAFDERR |
| 1,239.6156 | 1,239.6178 | −1.79 | NP | 66–75 | MoxVLSAFDERR |
| 1,323.6223 | 1,323.6252 | −2.16 | NP | 163–174 | MCSLMQGSTLPR |
| 1,334.6745 | 1,334.6748 | −0.2 | NP | 294–305 | py-EGYSLVGIDPFR |
| 1,339.6172 | 1,339.6175 | −0.2 | NP | 163–174 | MoxCSLMQGSTLPR |
| 1,352.685 | 1,352.6878 | −2.04 | NP | 294–305 | EGYSLVGIDPFR |
| 1,420.7688 | 1,420.772 | −2.27 | NP | 107–117 | ELILYDKEEIR |
| 1,482.7011 | 1,482.704 | −1.95 | NP | 423–436 | TTIMAAFNGNTEGR |
| 1,739.7845 | 1,739.7869 | −1.37 | NP | 447–461 | MMESARPEDVSFQGR |
| 1,755.7794 | 1,755.7838 | −2.49 | NP | 447–461 | MoxMESARPEDVSFQGR |
| 2,065.9943 | 2,066.0005 | −2.99 | NP | 244–261 | ESRNPGNAEFEDLTFLAR |
| 2,104.9472 | 2,104.946 | 0.58 | NP | 423–441 | SCLPACVYGPAVASGYDFER |
| 2,156.9519 | 2,156.9565 | −2.15 | NP | Sep-26 | SYEQMETDGERQNATEIR |
| 2,225.0773 | 2,225.0786 | −0.57 | NP | 417–436 | NLPFDRTTIMAAFNGNTEGR |
| 2,355.0808 | 2,355.086 | −2.2 | NP | 362–382 | GVQIASNENMoxETMESSTLELR |
| 3,245.4493 | 3,245.4584 | −2.81 | NP | 122–150 | QANNGDDATAGLTHMMIWHSNLNDATYQR |
| 3,438.6139 | 3,438.6104 | 1.01 | NP | 274–305 | SCLPACVYGPAVASGYDFEREGYSLVGIDPFR |
| 399.2105 | 399.2104 | 0.13 | M1 | 161–163 | SHR |
| 473.2836 | 473.2842 | −1.23 | M1 | 73–76 | GLQR |
| 555.2639 | 555.2647 | −1.36 | M1 | 175–178 | HENR |
| 687.379 | 687.3803 | −1.93 | M1 | 22–27 | AEIAQR |
| 846.3967 | 846.3984 | −2.05 | M1 | 211–217 | py-QMVQAMR |
| 849.4075 | 849.4089 | −1.63 | M1 | 244–250 | MGVQMQR |
| 863.4232 | 863.4251 | −2.24 | M1 | 211–217 | QMVQAMR |
| 865.4024 | 865.4036 | −1.37 | M1 | 244–250 | MoxGVQMQR |
| 879.4181 | 879.4204 | −2.66 | M1 | 211–217 | QMoxVQAMR |
| 902.4736 | 902.4747 | −1.22 | M1 | 106–113 | EITFHGAK |
| 921.5079 | 921.5101 | −2.34 | M1 | 179–187 | MVLASTTAK |
| 1,005.5086 | 1,005.5105 | −1.86 | M1 | 243–250 | RMGVQMQR |
| 1,125.6996 | 1,125.7013 | −1.53 | M1 | 48–57 | TRPILSPLTK |
| 1,256.6673 | 1,256.6692 | −1.48 | M1 | 164–174 | py-QMVTTTNPLIR |
| 1,272.6622 | 1,272.6647 | −1.93 | M1 | 164–174 | py-QMoxVTTTNPLIR |
| 1,273.6938 | 1,273.697 | −2.48 | M1 | 164–174 | QMVTTTNPLIR |
| 1,289.6887 | 1,289.6882 | 0.42 | M1 | 164–174 | QMoxVTTTNPLIR |
| 1,490.7565 | 1,490.76 | −2.35 | M1 | 36–47 | NTDLEVLMEWLK |
| 1,604.8396 | 1,604.8424 | −1.72 | M1 | 231–243 | NDLLENLQAYQKR |
| 1,635.911 | 1,635.9138 | −1.69 | M1 | 58–72 | GILGFVFTLTVPSER |
| 1,669.8808 | 1,669.8829 | −1.26 | M1 | 161–174 | SHRQMoxVTTTNPLIR |
| 1,809.9394 | 1,809.9342 | 2.87 | M1 | 164–178 | QMVTTTNPLIRHENR |
| 2,003.9358 | 2,003.9314 | 2.17 | M1 | 78–95 | RFVQNALNGNGDPNNMDK |
| 2,219.084 | 2,219.0865 | −1.11 | M1 | 114–134 | EISLSYSAGALASCMGLIYNR |
| 2,350.2005 | 2,350.2068 | −2.69 | M1 | 28–47 | LEDVFAGKNTDLEVLMEWLK |
| 2,685.3848 | 2,685.3847 | 0.03 | M1 | 218–242 | TIGTHPSSSAGLKNDLLENLQAYQK |
| 2,737.2748 | 2,737.2733 | 0.55 | M1 | 135–160 | MGAVTTEVAFGLVCATCEQIADSQHR |
a
“mono” indicates monoisotopic values.