TABLE 4.

Peptides identified in a MALDI-FT-ICR mass spectrum (Fig. 5) of a whole-virus digest of a cell-cultured clinical specimen containing a type A H3N2 seasonal influenza strain^a

[M+H]+ m/z mono.		Difference (ppm)	Viral protein	Residues	Sequence	Po	Signature or indicator	Period-specific signature	Bayes
Theoretical	Experimental
846.3961	846.3971	−1.23	M1	211–217	py-QMVQAMR	0.91			S
849.4070	849.4086	−1.94	M1	244–250	MGVQMQR	0.97			S
852.4363	852.4377	−1.70	NP	389–395	SGYWAIR	0.72	I	S	S
863.4226	863.4246	−2.31	M1	211–217	QMVQAMR	0.91
880.4345	880.4353	−0.86	HA	317–324	ITYGACPR	0.92	S		S
964.4887	964.4900	−1.34	HA	100–106	WDLFVER	0.97		S
1,177.5861	1,177.5864	−0.24	NP	206–214	GINDRNFWR	0.93			S
1,191.6150	1,191.6162	−0.98	NP	443–452	TSDMRAEIIR	0.74			S
1,219.6537	1,219.6535	0.15	NP	185–195	GIGTMVMELIR	0.91		S
1,223.6201	1,223.6193	0.67	NP	72–81	MVLSAFDERR	0.98			S
1,235.6486	1,235.6493	−0.53	NP	191–201	GIGTMoxVMELIR	0.84			S
1,323.6218	1,323.6224	−0.47	NP	169–180	MCSLMQGSTLPR	1.00
1,406.7526	1,406.7523	0.19	NP	113–123	ELVLYDKEEIR	0.92
1,455.6897	1,455.6883	0.96	NP	423–436	STIMAAFTGNTEGR	0.90		S
1,510.7029	1,510.7010	1.23	NP	453–465	MMEGAKPEEVSFR	0.79	I
1,620.8275	1,620.8299	−1.49	NP	236–249	FQTAAQRAMVDQVR	0.64
1,635.9105	1,635.9085	1.20	M1	58–72	GILGFVFTLTVPSER	0.97
1,663.8398	1,663.8398	0.03	NP	401–416	ASAGQTSVQPTFSVQR	0.96		S
1,671.8701	1,671.8700	0.04	NP	253–267	NPGNAEIEDLIFLAR	0.81			S
2,044.0458	2,044.0431	1.32	NP	250–267	ESRNPGNAEIEDLIFLAR	0.78			S
2,266.0437	2,266.0423	0.62	NP	362–382	GVQIASNENMDNMGSSTLELR	0.95		S
2,266.0438	2,266.0423	0.67	NP	368–388	GVQIASNENMDNMGSSTLELR	0.63			S
2,413.0428	2,413.0457	−1.19	M1	188–210	AMoxEQMAGSSEQAAEAMEIASQAR	0.72

^aS, signature peptide; I, indicator peptide; “mono.” indicates monoisotopic values.