TABLE 5.
Peptides identified in a MALDI-FT-ICR mass spectrum (Fig. 6) of a whole virus digest of a cell-cultured clinical specimen containing a type B influenza straina
| [M+H]+
m/z mono. |
Difference (ppm) | Viral protein | Residues | Sequence | Po | Signature or indicator | Period-specific signature | Bayes | |
|---|---|---|---|---|---|---|---|---|---|
| Theoretical | Experimental | ||||||||
| 731.4199 | 731.4209 | −1.42 | HA | 352–357 | YRPPAK | 0.96 | S | S | S |
| 771.4723 | 771.4733 | −1.31 | M1 | 95–101 | GLILAER | 1.00 | S | ||
| 778.3665 | 778.3663 | 0.20 | HA | 28–294 | VWCASGR | 0.97 | S | S | S |
| 816.4363 | 816.4370 | −0.90 | HA | 54–60 | SHFANLK | 0.67 | S | ||
| 836.4989 | 836.4987 | 0.20 | HA | 121–127 | py-QLPNLLR | 0.95 | S | ||
| 853.5254 | 853.5258 | −0.48 | HA | 121–127 | QLPNLLR | 0.99 | S | S | S |
| 939.4893 | 939.4893 | 0.00 | HA | 273–280 | TGTITYQR | 0.99 | S | ||
| 996.5513 | 996.5509 | 0.39 | NP | 163–170 | TIYFSPIR | 0.93 | S | S | |
| 1,043.5844 | 1,043.5834 | 0.91 | NP | 246–256 | GGGTLVAEAIR | 1.00 | S | ||
| 1,057.6000 | 1,057.5995 | 0.45 | NP | 295–304 | ALVDQVIGSR | 1.00 | S | ||
| 1,100.5922 | 1,100.5910 | 1.06 | HA | 332–341 | AIGNCPIWVK | 0.97 | S | ||
| 1,102.6870 | 1,102.6866 | 0.37 | NP | 332–341 | VVLPISIYAK | 1.00 | S | S | S |
| 1,149.5874 | 1,149.5863 | 0.96 | NP | 439–447 | LQFWAPMTR | 1.00 | S | S | S |
| 1,165.5823 | 1,165.5806 | 1.00 | NP | 439–447 | LQFWAPMoxTR | 1.44 | |||
| 1,209.6051 | 1,209.6047 | 0.35 | NP | 540–549 | py-QTIPNFFFGR | 0.96 | S | ||
| 1,226.6317 | 1,226.6301 | 1.32 | NP | 540–549 | QTIPNFFFGR | 0.96 | S | S | S |
| 1,245.6685 | 1,245.6658 | 2.13 | HA | 413–423 | NLNSLSELEVK | 0.97 | S | ||
| 1,259.7140 | 1,259.7091 | 3.88 | NP | 320–331 | SMVVVRPSVASK | 1.00 | S | ||
| 1,264.6757 | 1,264.6744 | 1.00 | NP | 106–116 | NLIQNAHAVER | 0.95 | S | ||
| 1,280.6270 | 1,280.6278 | −0.66 | HA | 321–331 | SKPYYTGEHAK | 0.96 | S | S | S |
| 1,359.6436 | 1,359.6412 | 1.73 | NP | 86–96 | LGEFYNQMMVK | 1.00 | S | S | S |
| 1,418.8002 | 1,418.7981 | 1.46 | NP | 399–411 | VLSALTGTEFKPR | 0.96 | S | S | S |
| 1,441.8009 | 1,441.7976 | 2.29 | M1 | 201–213 | LAEELQSNIGVLR | 1.00 | S | S | S |
| 1,465.7209 | 1,465.7184 | 1.74 | M1 | 36–47 | EFDLDSALEWIK | 1.00 | S | ||
| 1,581.7941 | 1,581.7903 | 2.38 | HA | 300–314 | GSLPLIGEADCLHEK | 0.97 | S | S | S |
| 1,610.8748 | 1,610.8706 | 2.58 | NP | 305–319 | NPGIADIEDLTLLAR | 1.00 | S | S | S |
a
S, signature peptide; “mono.” indicates monoisotopic values.