TABLE 3.
Summary of sensitivity and specificity of the HT-J test relative to liquid radiometric (Bactec) culture with different scenarios for conduct of the HT-J test or test result reporting
| Scenario | Description | Sheep |
Cattle |
||
|---|---|---|---|---|---|
| Sensitivitya | Specificityb | Sensitivitya | Specificityb | ||
| 1c | DNA extracts tested in duplicate. Samples with one positive replicate and one negative replicate were classified as negative | 83.8 | 98.9 | 60.4 | 99.6 |
| 2 | DNA extracts tested in duplicate. Samples with one positive replicate and one negative replicate were classified as positive | 85.6 | 97.8 | 63.1 | 97.6 |
| 3 | DNA extracts tested in single wells | 84.7–85.7 | 97.8–98.9 | 61.3–62.2 | 98.5–98.7 |
| 4 | DNA extracts tested in duplicate. Average DNA quantity of the replicates used to determine result. | 85.6 | 98.9 | 62.2 | 98.7 |
a
Proportion (%) of culture-positive samples from exposed herds/flocks that tested positive in HT-J.
b
Proportion (%) of culture-negative samples from unexposed herds/flocks that tested negative in HT-J.
c
Scenario 1 was the format of test conduct and result reporting criteria that were applied for the HT-J test.