TABLE 1.
Identification results obtained using rpoB gene sequencing and three commercial systems, antimicrobial susceptibility, and genotyping results for the 11 bloodstream isolates of Acinetobacter GS 13BJ/14TU
| Isolate no. | Date of isolation (yr-mo-day) | Species identified by: |
Antimicrobial susceptibilityb | MLSTc |
PFGEg pattern | |||||
|---|---|---|---|---|---|---|---|---|---|---|
| rpoB gene sequence analysisa | Vitek 2a | MicroScana | API 20 NE |
|||||||
| Resulta | Code no. | Allele profiled | STf | |||||||
| 1 | 2003-10-07 | Acinetobacter GS 13BJ/14TU (97) | A. baumannii complex (95) | A. baumannii/haemolyticus (99.99) | A. haemolyticus (95.2) | 0010053 | All S, except colistin (R) | 18-22-42e −11-21-14-21 | 198e | P1 |
| 2 | 2004-03-15 | Acinetobacter GS 13BJ/14TU (100) | A. baumannii complex (96) | A. lwoffii (99.99) | A. haemolyticus (99.9) | 0010051 | All S, except colistin (R) | 21-25-24-15-24-17-24 | 69 | P2 |
| 3 | 2005-10-18 | Acinetobacter GS 13BJ/14TU (97) | A. baumannii complex (99) | A. baumannii/haemolyticus (98.18) | A. haemolyticus (95.2) | 0010053 | All S, except tetracycline (R) and colistin (R) | 18-22-42e-11-21-14-21 | 198e | P3 |
| 4 | 2008-08-03 | Acinetobacter GS 13BJ/14TU (100) | A. baumannii complex (98) | A. baumannii/haemolyticus (98.18) | A. haemolyticus (95.2) | 0010053 | All S, except colistin (R) | 21-25-24-28e-46e-17-24 | 200e | P4 |
| 5 | 2009-07-24 | Acinetobacter GS 13BJ/14TU (99) | A. baumannii complex (99) | A. baumannii/haemolyticus (98.18) | A. haemolyticus (95.2) | 0010053 | All S, except colistin (R) | 21-25-42e-15-24-17-24 | 201e | P5 |
| 6 | 2009-10-01 | Acinetobacter GS 13BJ/14TU (97) | A. baumannii complex (98) | A. baumannii/haemolyticus (98.18) | A. haemolyticus (99.9) | 0010051 | All S, except colistin (R) | 18-22-42e-11-21-14-21 | 198e | P6 |
| 7 | 2009-11-03 | Acinetobacter GS 13BJ/14TU (97) | A. baumannii complex (99) | A. baumannii/haemolyticus (66.99) | A. haemolyticus (95.2) | 0010053 | All S, except colistin (R) | 18-22-42e-11-21-14-21 | 198e | P7 |
| 8 | 2010-05-11 | Acinetobacter GS 13BJ/14TU (99) | A. baumannii complex (99) | A. baumannii/haemolyticus (95.16) | A. haemolyticus (95.2) | 0010053 | All S, except colistin (R) | 18-22-42e-11-21-14-24 | 199e | P8 |
| 9 | 2010-07-03 | Acinetobacter GS 13BJ/14TU (97) | A. lwoffii (97) | A. baumannii/haemolyticus (98.18) | A. haemolyticus (95.2) | 0010053 | All S, except colistin (R) | 18-22-42e-11-21-14-21 | 198e | P9 |
| 10 | 2011-07-11 | Acinetobacter GS 13BJ/14TU (97) | A. baumannii complex (99) | A. baumannii/haemolyticus (98.18) | A. haemolyticus (95.2) | 0010053 | All S, except ciprofloxacin (I) and colistin (R) | 18-22-42e-11-21-14-21 | 198e | P10 |
| 11 | 2011-08-09 | Acinetobacter GS 13BJ/14TU (99) | A. baumannii complex (99) | P. stuartii (75.88) | A. haemolyticus (95.2) | 0010053 | All S, except colistin (R) | 21-25-24-28e-46e-17-24 | 200e | P11 |
| ATCC 17905 | Acinetobacter GS 13BJ/14TU (100) | A. baumannii complex (98) | A. baumannii/haemolyticus (98.18) | A. haemolyticus (95.2) | 0010053 | All S, except colistin (R) | 21-25-24-15-24-17-24 | 69 | P12 | |
Numbers in parentheses are the probabilities of correct identification (as percentages).
Antimicrobial susceptibility testing was performed using the disk diffusion method for ampicillin-sulbactam, piperacillin-tazobactam, ticarcillin-clavulanic acid, ceftazidime, cefepime, ceftriaxone, imipenem, meropenem, gentamicin, tobramycin, amikacin, tetracycline, and ciprofloxacin; colistin susceptibility was determined using the agar dilution method. S, susceptible; I, intermediate; R, resistant.
MLST, multilocus sequence typing.
Allele profiles for cpn60-fusA-gltA-pyrG-recA-rplB-rpoB.
Three new alleles and four new STs identified in this study were added to the Institut Pasteur MLST database (http://www.pasteur.fr/mlst).
ST, sequence type.
PFGE, pulsed-field gel electrophoresis.