FIG 5.

NMR data of 11Av PS, revealing biochemical signatures of both 11A and 11E PSs. (A and B) The anomeric (A) and methyl (B) regions of the ¹H NMR spectra of native capsule PSs purified from strains 3102-06 (11Av), MNZ2293-C (11Av), MNZ264 (11E), and MNZ2293-A (11A). O-Acetylation-associated signals discussed in the text are labeled with their respective chemical shift values (in ppm). *, hydrolysis-resistant signals arising from contaminating teichoic acid. (C) Select regions of the 2D ¹H-¹³C HMQC NMR spectra of native capsule PSs purified from strains MNZ2293-A, 3102-06, and MNZ264. Signals are labeled with their corresponding shift values (¹H/¹³C, in ppm). In A to C, all spectra are normalized according to the signal intensity arising from the H2 proton of βGlc at 3.36 ppm (see Fig. 4). #, signals present in 11E and 11Av spectra that are grossly absent in 11A spectra.