Figure 2.

Immunohistochemistry (IHC) for TIMP-1 performed on parallel tissue sections of hyperplastic prostate (A-C) and normal colon (D-F) with the VT7 MAb (1 μg/mL) using different IHC protocols concerning antigen retrieval. (A,D) No antigen retrieval. (B,E) Proteolytic pretreatment (proteinase K). (C) Heat-induced epitope retrieval (HIER) in 10 mM citrate buffer, pH 6. (F) HIER in target retrieval solution, pH 6.1. Strong immunoreactivity is obtained in both tissues with HIER pretreatment (C,F), and weak staining is also seen without pretreatment (A,D). In prostate, staining is observed in the epithelial cells (e) of A,C with no staining in the stroma (s). In normal colon mucosa, immunoreactivity is seen in neuroendocrine cells (arrows in D,F). Bars: A-C = 50 μm; D-F = 20 μm.