Table 2.

Primer sets used for RT-PCR of MCT1, MCT2, and MCT4 ^a

MCT	Primers	Sequence	Total bp	Coding bases
MCT1	P1	GGAGGTCTTGGGCTTGCCTTCAACT	799	358-382
	P2	GGACTCTTTGCACCTTTGGTGT	331	826-847
	P3	GGATTCTGTGTCTATGCGGGATTC	94	1063-1086
	RP1	CAACAAGGTCCATCAATGTTTCAA		1157-1134
MCT2	P1	CGGGCGCCCACCCTGCGCCAGAGACCAG	650	(−161)-(−134)
	P2	TAATTGGCAAATACTTCTATAGGAAGCG	636	401-428
	RP1	AGGAGCCAATGAACTTAAGAAAACAGGA		489-462
	RP2	AATACAGCATATAATACCAGGCTTGT		1037-1012
MCT4	P1	GGCGAGAGGCGGGCTGAGGCGGCCCAG	582	(−62)-(−36)
	P2	CCCTGTCTTCCTGTGTGCCCTGAG	601	468-492
	P3	CCATCGTGGGCACCCACAAGTTCT	419	1034-1057
	RP1	AGCGGTCCTGCAGCAGCTGCCCCAGCG		520-594
	RP2	CAATGGCACTGGAGAACTTGTGGG		1069-1046
	RP3	AAGCGTTGCCGGCTTCTGTACCTC		1453-1430

^aThe correspondingly numbered P and RP were used as primer pairs for each amplification except for MCT1, where one RP was used for all. P is a forward primer (5′ × 3′) and RP is a reverse primer (3′ × 5′). The coding bases count from the start codon's first nucleotide. Nucleotides in parentheses precede the coding region counting 5′(−) backward before the first coding base. Total bp listed in column 4.