TABLE 1.
Gene-specific primer pairs used for reverse transcription-quantitative PCR assay
| Gene | Primer name | Nucleotide sequence (5′→3′) |
|---|---|---|
| NCU09686 (ccg-8) | QN9686F | AAGGTGGCTCTCTCCTTTA |
| QN9686R | GGTCATTTGGTTCATCTTCTTG | |
| NCU02624 (erg11) | QN2624F | CCGCCATTGTCAAGGAAA |
| QN2624R | ACGTGATCGGTCGGAATA | |
| NCU05591 (cdr4) | QN5591F | ACGCTTTGGAAATGGATGGTGACG |
| QN5591R | ATGAACAAGGCGACGGAAATGCAG | |
| NCU06666 (ino1) | QN6666F | CACACCGTTGTGATCAAGTA |
| QN6666R | CACGTTGAAGAGCGAGATG | |
| NCU06317 (kts-1) | QN6317F | CACTACCACCACCAACAAG |
| QN6317R | GGACCAGCATGTGCAATA | |
| NCU01386 (kts-2) | QN1386F | ATGGCACCCTTTGTGATG |
| QN1386R | CAGTCCATCTCTCTTGGAAAC | |
| NCU09915 (fsd-1/ndt80) | QN9915F | ATCCGAGAGGTGGCTATG |
| QN9915R | GTAGATCGTTGCAGGGAAAT | |
| NCU09691 (kts-3) | QN9691F | GATTGCGACCCGAAGAAG |
| QN9691R | GGATCATCCACACAAGTCAG | |
| NCU02084 (arginase) | QN2084F | GATAGTCACATCGACACATGG |
| QN2084R | ATGGAGGTGTTGTGGATTAAG | |
| NCU09635 (kts-4) | QN9635F | CATCCCTCCTAACAACAAGAC |
| QN9635R | CCGTTGGCATAATTGACATAAG | |
| NCU01315 (gt41-1) | QN1315F | CTGATTGCTGGCGATGATTA |
| QN1315R | CACCACCAGCACCATTATAC | |
| NCU08957 (kts-5) | QN8957F | CTCTGACATCTCGGACTTTG |
| QN8957R | TTGGAGGAGGAGGAAGATT | |
| NCU01633 (hxt13) | QN1633F | GACTCCATTGGCTACTTCTATG |
| QN1633R | TACATGGTCTCGATCTCCTC | |
| NCU00247 (kts-6) | QN0247F | CGCCTTCTTCAGCTTCTTT |
| QN0247R | GATGATGGTGGGATGAATGAG | |
| NCU01302 (hydrolase) | QN1302F | CATTCCCTACCAAGAGATCAAG |
| QN1302R | GTTGCAGATAGGACGAGTATG | |
| NCU00611 (kts-7) | QN0611F | CATATCTCCATGCCACTCTTG |
| QN0611R | CAAGGAGTTCAGCTGGTAAG | |
| NCU00248 (kts-8) | QN0248F | GAGGTAGGAGGAACGACAT |
| QN0248R | GGGCATCCCTCTGATAAGTA | |
| NCU04334 (cpn10) | QN4334F | CCCGAGTCCTCCGTTAAG |
| QN4334R | GCCGTACTGAGGGATAAGAA | |
| NCU04923 (gcy-1) | QN4923F | GAGAGCAACTTCCAGATTCC |
| QN4923R | CCAGACATCGTAGCCAAAG | |
| β-tub | QbtubF | CCCAAGAACATGATGGCTGCTTCT |
| QbtubR | TTGTTCTGAACGTTGCGCATCTGG |