TABLE 2.
Locus and/or categoryb | Gene | Function | TPM in WT | TPM in WT(k) | Fold change [WT(k)/WT] | P value | TPM in Δccg-8 | TPM in Δccg-8(k) | Fold change [Δccg-8(k)/Δccg-8] | P value | Fold change [Δccg-8(k)/WT(k)] | Sensitivity to ketoconazole compared to WT's |
---|---|---|---|---|---|---|---|---|---|---|---|---|
Phospholipid synthesis pathways | ||||||||||||
NCU06666 | ino1 | Inositol-3-phosphate synthase | 38.86 | 21.55 | 0.55 | 2.65E−05 | 180.9 | 17.25 | 0.09 | 4.7E−117 | 0.80 | Similar |
NCU08045 | cho2 | Phosphatidylethanolamine N-methyltransferase | 11.22 | 6.16 | 0.55 | 0.022589 | 110 | 27.06 | 0.25 | 3.34E−41 | 4.39 | NA |
NCU03695 | psd1 | Phosphatidylserine decarboxylase proenzyme 1 | 28.78 | 51.28 | 1.78 | 1.87E−14 | 69.7 | 115.08 | 1.65 | 7.63E−10 | 2.26 | Similar |
NCU02381 | cho1 | Phosphatidylserine synthase | 18.13 | 8.12 | 0.45 | 0.000217 | 29.96 | 13.08 | 0.44 | 1.91E−06 | 1.61 | NA |
NCU09643 | cds1 | Phosphatidate cytidylyltransferase | 17.56 | 13.43 | 0.76 | 0.165267 | 24.02 | 18.73 | 0.78 | 0.138875 | 1.39 | NA |
Kinases participating in Opi1p (CCG-8 homolog in S. cerevisiae) phosphorylation | ||||||||||||
NCU06240 | pkac-1 | Catalytic subunit protein kinase A | 26.48 | 24.63 | 0.93 | 0.6266 | 40.04 | 9.81 | 0.25 | 5.40E−16 | 0.40 | Similar |
NCU06544 | pkc | Protein kinase C | 24.75 | 67.16 | 2.71 | 0 | 52.79 | 24.98 | 0.47 | 5.28E−09 | 0.37 | Similar |
NCU03124 | ck2 | Catalytic subunit of protein kinase CK2 | 68.21 | 155.87 | 2.29 | 1.61E−13 | 52.2 | 60.07 | 1.15 | 0.173376 | 0.39 | Similar |
Target enzyme of azoles and multidrug transporters | ||||||||||||
NCU02624 | erg11 | Cytochrome P450 lanosterol 14a-demethylase | 69.08 | 676.66 | 9.80 | 0 | 113.9 | 254.84 | 2.24 | 4.01E−13 | 0.38 | NA |
NCU05591 | cdr4 | ABC transporter CDR4 | 3.74 | 259.97 | 69.51 | 2.17E−07 | 4.75 | 54.42 | 11.46 | 4.10E−08 | 0.21 | Hypersensitive |
NCU04161 | Multidrug resistance-associated protein 5 | 2.59 | 20.99 | 8.10 | 2.57E−06 | 4.75 | 6.84 | 1.44 | 0.264586 | 0.33 | Similar | |
Stress responses | ||||||||||||
NCU01499 | Related to hsp70 | 16.12 | 39.74 | 2.47 | 1.97E−09 | 35.29 | 147.49 | 4.18 | 0 | 3.71 | Similar | |
NCU06317 | kts-1 | Stress response RCI peptide | 123.19 | 1142.04 | 9.27 | 1.56E−13 | 323.3 | 412.45 | 1.28 | 1.56E−09 | 0.36 | Hypersensitive |
NCU09873 | pck-1/acu-6 | Phosphoenolpyruvate carboxykinase | 4.89 | 31.06 | 6.35 | 1.68E−08 | 300.1 | 275.96 | 0.92 | 0.064493 | 8.88 | Similar |
NCU00355 | cat-3 | Catalase-3 | 135.85 | 252.42 | 1.86 | 0 | 54.28 | 24.98 | 0.46 | 1.08E−09 | 0.10 | Similar |
Transcription factors | ||||||||||||
NCU09686 | ccg-8 | Clock-controlled protein 8 | 33.97 | 127.89 | 3.76 | 3.94E−13 | 0 | 0 | Hypersensitive | |||
NCU01386 | kts-2 | Zn(II)2Cys6 domain-containing protein | 0.58 | 26.86 | 46.31 | 0.000112 | 1.78 | 5.95 | 3.34 | 0.00582 | 0.22 | Hypersensitive |
NCU01629 | C2H2 finger domain-containing protein | 4.03 | 53.45 | 13.26 | 1.15E−07 | 28.77 | 25.57 | 0.89 | 0.427838 | 0.48 | Similar | |
NCU05536 | Zn(II)2Cys6 domain-containing protein | 6.04 | 48.97 | 8.11 | 1.25E−09 | 6.23 | 8.33 | 1.34 | 0.317684 | 0.17 | Similar | |
NCU09496 | C2H2 transcription factor | 10.07 | 97.94 | 9.73 | 2.66E−13 | 37.37 | 47.58 | 1.27 | 0.042249 | 0.49 | NA | |
NCU04022 | C2H2 finger domain-containing protein | 12.66 | 120.89 | 9.55 | 6.62E−14 | 33.22 | 29.44 | 0.89 | 0.38236 | 0.24 | NA | |
NCU09915 | fsd-1/ndt80 | Female sexual development-1 protein | 7.77 | 46.17 | 5.94 | 2.51E−11 | 46.56 | 21.41 | 0.46 | 1.60E−08 | 0.46 | Hypersensitive |
WT, wild type; WT(k) or Δccg-8(k), wild type or Δccg-8 mutant treated with ketoconazole, respectively; TPM, number of locus tags per million; NA, the mutant was not available.
Locus numbers and function were annotated according to the N. crassa genome assembly (http://www.broadinstitute.org/annotation/genome/neurospora/).